Total salt-soluble protein extract of lentil seeds was fractionated with differenct concentration intervals of ammonium sulphate. Fractions were separated by chromatography on hydroxylapatite column. Chromatographic fractions were analysed by electrophoresis on polyacrylamide gel.The seeds contained a highly heterogeneous system of individual proteins. Fraction 0-30 was composed of minor protein components and nucleic acids. Fraction 30-60 was represented by primary and secondary globulins. The fraction which preciptated with ammonium sulphate of above 60% concentration was dominated by vicilin and legumin. Which were inseparable on hydroxylapatite column and had maximum elution at 0.29 M phosphate buffer.
S H SIDDIQUI ,
You can use below filter to find your desired issue