Theoplline, caffeine and their important metabolites were extracted from human plasma using newly developed solid support liquid/liquid extraction tubes. These substances were separated on a 3 mum ODS (250 x 4.6 mm i.d) column with a mobile phase gradient and detected with a UV diode array detector set at two wave lengths of 270 and 290 nm. The normal ranges for the plasma drug/metabolite molar ratios have been determined.
A RAZAK SOMO ,S ALI ARBANI ,M Y KHAHAWER ,M A CHIPPA ,G ALI QURSHI ,
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